In life, the Lesbian Ghosts were patients at the Vannacutt Psychiatric Institute for the Criminally Insane, and were also lovers. However, at some point, Dr. Vannacutt had them killed side-by-side via electrotherapy.
After the two lesbian patients were killed in the asylum, the Baphomet Idol kept their ghosts bound to the building, along with the ghosts of everyone else who died in the facility.Harue comes across and investigates the electrotherapy room where the two lesbian patients died, the two ghosts appear to and seduce her. The Lesbian Ghosts begin to make love to Harue, when Harue sees through a kiss from one of the ghosts a vision of how they died; causing the Lesbian Ghosts to transform into demonic forms. The sight of the Lesbian Ghosts' monstrous forms snaps Harue back to her senses and causes her to flee in disgust. The two ghosts then begin kissing each other.
The Lesbian Ghosts were presumably freed from the house hours later when Ariel Wolfe removed the Baphomet Idol from the building.